LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1562..1658)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1673..1692)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1700..1716
                     /note="M13 Reverse priming site"
     gene            1829..2638
                     /note="Kanamycin resistance gene"
     rep_origin      2759..3432
                     /note="pUC origin"
     vector          join(1570..3435,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1555
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:104794 IMAGE:5930073"
                     /tissue_type="Lymph, lymphoma"
                     /clone_lib="NIH_MGC_99"
                     /lab_host="DH10B"
                     /note="Vector: pOTB7"
     gene            692..1555
                     /gene="MFNG"
                     /db_xref="GeneID:4242"
                     /db_xref="HGNC:7038"
                     /db_xref="MIM:602577"
     CDS             692..1555
                     /dnas_title="MFNG O-fucosylpeptide
                     3-beta-N-acetylglucosaminyltransferase"
                     /gene="MFNG"
                     /codon_start=1
                     /product="MFNG O-fucosylpeptide
                     3-beta-N-acetylglucosaminyltransferase"
                     /protein_id="AAH94814.1"
                     /db_xref="GI:63102281"
                     /db_xref="GeneID:4242"
                     /db_xref="HGNC:7038"
                     /db_xref="MIM:602577"
                     /translation="MQCRLPRGLAGALLTLLCMGLLCLRYHLNLSPQRVQGTPELSQP
                     NPGPPKLQLHDVFIAVKTTRAFHRLRLELLLDTWVSRTREQTFVFTDSPDKGLQERLG
                     SHLVVTNCSAEHSHPALSCKMAAEFDTFLASGLRWFCHVDDDNYVNPRALLQLLRAFP
                     LARDVYVGRPSLNRPIHASEPQPHNRTRLVQFWFATGGAGFCINRKLALKMAPWASGS
                     RFMDTSALIRLPDDCTMGYIIECKLGGRLQPSPLFHSHLETLQLLRTAQLPEQVTLSY
                     GVFEGKLNVIKLQGPFSPEEDPSRFRSLHCLLYPDTPWCPQLGAR"
     misc_feature    692..1555
                     /note="MFNG coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg ccgggtacaa gggacccccg agctgagcca
        721 gccgaacccg gggcccccta agctacagct acacgatgtc ttcattgcag tgaagacgac
        781 ccgggctttc caccgcttgc gcctggagct gctgcttgac acgtgggttt ccaggaccag
        841 ggaacagaca tttgtcttca ccgacagccc agacaaaggc ctccaggaga gactggggtc
        901 ccaccttgtg gtcaccaact gctccgcgga acacagccac ccagctctgt cctgcaagat
        961 ggctgctgag ttcgacacct tcttggccag tgggcttagg tggttctgcc atgtggacga
       1021 tgacaactat gtgaacccaa gggcgctgct gcagcttctg agagccttcc cgctggcccg
       1081 cgacgtctat gtgggaaggc ccagcctgaa ccggcccatc catgcctcag agccacagcc
       1141 ccacaaccgc acgaggctgg tacagttctg gtttgccact gggggtgctg gcttctgcat
       1201 caatcgcaaa ctggctttga agatggctcc gtgggccagt ggctcccgtt tcatggacac
       1261 atctgctctc atccggctgc ctgatgactg caccatgggc tatatcattg agtgcaagct
       1321 gggcggccgc ctgcagccca gccccctctt tcactcccac ctggagaccc tgcagctgct
       1381 gaggactgca cagctcccag aacaggtcac cctcagctac ggtgtctttg aggggaaact
       1441 caacgtcatt aagctacagg gccccttctc cccggaggag gacccctcca gatttcgctc
       1501 cctccattgt ctgctctatc cagatacacc ctggtgtccc cagctgggtg cccgaTAGGA
       1561 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA
       1621 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA
       1681 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA
       1741 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC
       1801 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC
       1861 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT
       1921 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT
       1981 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA
       2041 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA
       2101 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA
       2161 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC
       2221 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA
       2281 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG
       2341 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT
       2401 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG
       2461 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG
       2521 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT
       2581 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC
       2641 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC
       2701 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC
       2761 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
       2821 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
       2881 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT
       2941 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
       3001 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
       3061 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
       3121 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
       3181 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
       3241 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
       3301 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
       3361 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
       3421 TTTTGCTCAC ATGTT
//
